The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Plus Prestained Protein Ladder in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That's why we're pleased to launch the color-enhanced Blu10 Plus  prestained protein standards.

As you may see the comparison data shown below, Blu10 Plus shows a better resolution than the Blu12 & Blu10  for the protein bands from 95 kDa to 270 kDa.

Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 10μl

Required materials but not provided:

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge

► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.
► Higher Added Value: Tri-colors and 10 bands with premium quality.
► Better performance for higher molecular weight proteins.
► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system.
► Enhanced transfer, wash, and stripping durabilities.

Storage Buffer : 
62.5 mM Tris-H₃PO₄ (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% glycerol.

Storage: 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Lane 1: PMB11-0500 (Bio-Helix) 5ul
Lane 2: PMB13-0500 (Bio-Helix) 5ul
Lane 3: PMB12-0500 (Bio-Helix) 5ul
Lane 4: PMB10-0500 (Bio-Helix) 5ul
Lane 5: PMB01-0500 (Bio-Helix) 5ul
Lane 6: PMB11-0500 (Bio-Helix) 5ul
Lane 7: PMB01-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.