The Blu10 Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 11 to 180 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa, serve as reference bands. The Blu10 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size, and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Prestained Protein Ladder in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer : 
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Storage : 
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Lane 1: PMB11-0500 (Bio-Helix) 5ul
Lane 2: PMB13-0500 (Bio-Helix) 5ul
Lane 3: PMB12-0500 (Bio-Helix) 5ul
Lane 4: PMB10-0500 (Bio-Helix) 5ul
Lane 5: PMB01-0500 (Bio-Helix) 5ul
Lane 6: PMB11-0500 (Bio-Helix) 5ul
Lane 7: PMB01-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge