This product is a ready-to-use PCR solution that comes pre-mixed with optimized concentrations of Taq DNA polymerase, dNTPs, Mg²⁺, reaction buffer, stabilizers, and PCR enhancers at a 2x concentration. It offers superior fidelity and amplification efficiency compared to standard PCR methods. Its key advantages include ease of use, high sensitivity, strong specificity, and excellent stability, all of which contribute to reducing potential human errors. The PCR product features a protruding "A" base at the 3' end, facilitating direct cloning into T vectors after purification. Additionally, the solution contains a red dye, allowing for direct gel loading post-PCR without the need for an additional loading buffer. It can also undergo purification for subsequent procedures such as restriction enzyme digestion, ligation, or fluorescent sequencing.

Figure 1. Amplification of Various Template Lengths using MB204-P040 with Different Template Sources and Concentrations (A) MB204-P040 (B) Competitor

Figure 2. Amplification Using MB204-P040 on Templates from Different Sources (A) MB204-P040 (B) Competitor