PureDireX Mbead Virus Genomic Nucleic Acid Kit (Magnetic Beads Based)

MBead Virus Genomic Nucleic Acid Kit was designed specifically for genomic DNA/RNA isolation from Virus samples. Its special buffer system will efficiently lyse cell and degrade protein, allowing for DNA/RNA to be easily bound by the surface of the magnetic beads. The other non-specific binding particles are removed with a wash buffer, and the genomic DNA/RNA is released from the beads by addition of a low ionic strength buffer and heat. Genomic DNA/RNA can be purified manually within 15 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

• Sample: Up to 300 μl of the virus sample
• Operation time: Within 10~15 minutes
• Applications: Restriction Enzyme Digestion, Southern Blotting, PCR and qPCR assays
• Storage: Room temperature

There was a study conducted with the following specifications:

Sample: EV71 Cell
Primer Mix Conc.: 200μM
Virus Stock Conc.: 4.9 x 10⁷ pfu/mL
Virus Input Conc.: 2.5 x 10⁶ pfu/mL
Procedure: Mbead Virus Genomic Nucleic Acid Kit (Mbeads Based) results after extracting virus from infected EV71 cell.